human leukemia t lymphocyte cells jurkat  (Procell Inc)

Journal: Frontiers in Pharmacology

Article Title: Obesity promotes immunotherapy efficacy by up-regulating the glycolytic-mediated histone lactacylation modification of CD8+ T cells

doi: 10.3389/fphar.2025.1533464

Figure Lengend Snippet: NSCLC tumor cells elevate PD-1 expression of T cells in a co-culture system. (A) Lactate levels in Jurkat cells when co-cultured with NCI-H23 cells at different concentrations of glucose in 0 h, 6 h, 12 h and 24 h; (B) HK1, (C) PDK1, (D) LDHA mRNA expressions by qPCR in NCI-H23 cells at different concentrations of glucose in 0 h, 6 h, 12 h and 24 h; (E) The protein levels of PD-1 in Jurkat cells when co-cultured with NCI-H23 cells at different concentrations of glucose in 0 h, 6 h, 12 h and 24 h.

Article Snippet: The NCI-H23 human NSCLC cell line, LLC mouse lung cancer cells and Jurkat human T lymphocytic leukemia cells were from ATCC and maintained in DMEM or RPMI 1640 (HyClone) supplemented with 10% FBS (Bioexplorer), 100 U/mL penicillin (Yeasen), and 100 U/mL streptomycin (Yeasen) at 37°C in a humidified incubator containing 5% CO 2 .

Techniques: Expressing, Co-Culture Assay, Cell Culture

Journal: Frontiers in Pharmacology

Article Title: Obesity promotes immunotherapy efficacy by up-regulating the glycolytic-mediated histone lactacylation modification of CD8+ T cells

doi: 10.3389/fphar.2025.1533464

Figure Lengend Snippet: Lactate released from tumor cells elevates T cell PD-1 expression. (A) Lactate levels in Jurkat cells when co-cultured with NCI-H23 cells treated with glucose, glucose + oxamic acid, glucose + rotenone; (B) PD-1 protein expression in Jurkat cells when co-cultured with NCI-H23 cells treated with glucose, glucose + oxamic acid, glucose + rotenone by Western blot; Comparison of PD-1 expression on mouse spleen CD8+ T cells cultured with or without lactate by (C) flow cytometry, (D) Western blot, and (E) qPCR; (F) Correlation analysis of PD-1 and MCT1 in CD8+ T cells in tumor microenvironment by single-cell sequencing data from ArrayExpress database (data number. E-MTAB-6149); (G) The mRNA expressions of PD-1 and MCT1 in different clusters of tumor infiltrating immune cells by scRNA-seq.

Article Snippet: The NCI-H23 human NSCLC cell line, LLC mouse lung cancer cells and Jurkat human T lymphocytic leukemia cells were from ATCC and maintained in DMEM or RPMI 1640 (HyClone) supplemented with 10% FBS (Bioexplorer), 100 U/mL penicillin (Yeasen), and 100 U/mL streptomycin (Yeasen) at 37°C in a humidified incubator containing 5% CO 2 .

Techniques: Expressing, Cell Culture, Western Blot, Comparison, Flow Cytometry, Sequencing